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Proceedings Paper

Biosensor of endotoxin and sepsis
Author(s): Yang Shao; Xiang Wang; Xi Wu; Wei Gao; Qing-hua He; Shaoxi Cai
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Paper Abstract

To investigate the relation between biosensor of endotoxin and endotoxin of plasma in sepsis. Method: biosensor of endotoxin was designed with technology of quartz crystal microbalance bioaffinity sensor ligand of endotoxin were immobilized by protein A conjugate. When a sample soliton of plasma containing endotoxin 0.01, 0.03, 0.06, 0.1, 0.5, 1.0Eu, treated with perchloric acid and injected into slot of quartz crystal surface respectively, the ligand was released from the surface of quartz crystal to form a more stable complex with endotoxin in solution. The endotoxin concentration corresponded to the weight change on the crystal surface, and caused change of frequency that occurred when desorbed. The result was biosensor of endotoxin might detect endotoxin of plasma in sepsis, measurements range between 0.05Eu and 0.5Eu in the stop flow mode, measurement range between 0.1Eu and 1Eu in the flow mode. The sensor of endotoxin could detect the endotoxin of plasm rapidly, and use for detection sepsis in clinically.

Paper Details

Date Published: 14 September 2001
PDF: 3 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440162
Show Author Affiliations
Yang Shao, Chongqing Univ. (China)
Xiang Wang, Chongqing Univ. (China)
Xi Wu, Chongqing Univ. (China)
Wei Gao, Chongqing Univ. (China)
Qing-hua He, Third Military Medical Univ. (China)
Shaoxi Cai, Chongqing Univ. (China)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

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