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Proceedings Paper

Development of an immunoassay for determination of 2,4-dichlorophenoxyacetic acid (2,4-D) based upon the recombinant Fab fragment of 2,4-D specific antibody
Author(s): Van Cuong Nguyen; Thi Dieu Thuy Nguyen; Hung Anh Dau; Thu Nga Tham; Dinh Thih Quyen; Till Bachmman; Rolf D. Schmid
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Paper Abstract

To develop an immunoassay and further an immunosensor for 2,4-D based upon recombinant antibody, the Fab fragments of 2,4-D specific antibody were expressed in E. coli. Western blotting analysis of the periplasmic cell fractions shown that under the non-reducing condition only a single protein band at a molecular mass of 45-kDa, corresponding to the whole Fab fragment was detected. Antigen binding activity for 2,4-D was found only in the extract of cells bearing the 2,4-D plasmid. An immunoassay based on the competitive reaction of 2,4-D and enzyme tracer with 2,4-D Fab fragments immobilized on micro titer plates via rabbit anti-mouse IgC was developed. Using this assay, 2,4-D could be detected at concentration range of 0.5 (mu) g/1 to 10(mu) g/1. The center point of the 2,4-D test was found at a concentration of 5 (mu) g/l. The assay was applied for detection of 2,4-D in spiked orange samples, resulting in recovery rate of 90 percent. The immunoassay could be applied to monitor human exposure to 2,4-D from contamination in fruit samples.

Paper Details

Date Published: 14 September 2001
PDF: 5 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440159
Show Author Affiliations
Van Cuong Nguyen, National Ctr. for Natural Science and Technology (Vietnam)
Thi Dieu Thuy Nguyen, National Ctr. for Natural Science and Technology (Vietnam)
Hung Anh Dau, National Ctr. for Natural Science and Technology (Vietnam)
Thu Nga Tham, National Ctr. for Natural Science and Technology (Vietnam)
Dinh Thih Quyen, National Ctr. for Natural Science and Technology (Vietnam)
Till Bachmman, Univ. Stuttgart (Germany)
Rolf D. Schmid, Univ. Stuttgart (Germany)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

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