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Proceedings Paper

New method for monitoring nitric oxide in vivo using microdialysis sampling and chemiluminescence reaction
Author(s): Dachun Yao; Nick P. Evmiridis; Yikai Zhou; Shunqing Xu; Huarong Zhou
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Paper Abstract

A new method employing a combination of micro dialysis sampling and chemiluminescence reaction was developed to monitor nitric oxide (NO) in vivo. A special probe was designed with an interference-free membrane to achieve a very high selectivity for NO. High sensitivity was achieved by optimizing the working system and improving the NO sampling time. This system was used in vivo to monitor blood and brain tissue in rats and rabbits. We have established that this system is sensitive enough to detect variations in NO production in difference physiological state. The system can detect NO in the linear range of 5nM-1(mu) M, with a detection limit of 1nM, and real NO concentrations in our experimental animals were found to be in the range of 1-5 nM or even less. Finally, the effects of body temperature, NO donors, Viagra, NO activators, NO cofactors, NO interference were investigated carefully in different physiological situations.

Paper Details

Date Published: 14 September 2001
PDF: 8 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440157
Show Author Affiliations
Dachun Yao, Univ. of Ioannina (Greece)
Nick P. Evmiridis, Univ. of Ioannina (Greece)
Yikai Zhou, Huazhong Univ. of Science and Technology (China)
Shunqing Xu, Huazhong Univ. of Science and Technology (China)
Huarong Zhou, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

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