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Proceedings Paper

Detection of DNA adducts by bioluminescence
Author(s): Shunqing Xu; Xianglin Tan; Qunfeng Yao; Min He; Yikai Zhou; Jian Chen
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Paper Abstract

Luminescent assay for detection ATP is very sensitive with limitation of 10-17 moles. ATP using styrene oxide as a model carcinogen we currently apply a luminescence technique to detect the very low levels of carcinogen-DNA adducts in vitro and in vivo. The bioluminescent assay of DNA adducts entails three consecutive steps: digestion of modified DNA to adducted dinucleoside monophosphate and normal nucleotide are hydrolyzed to nucleosides (N) by nuclease P1 and prostatic acid phosphomonesterase (PAP); incorporation of (gamma) -P of ATP into normal nucleoside(N); detection of consumption of ATP by luminescence. This assay does not require separate manipulation because of the selective property of nuclease P1. One fmol of carcinogen- DNA adducts was detected by luminescent assay. A good correlation between results of luminescent assay and 32P-postlabeling procedures has been observed. We detect 1 adduct in 108 nucleotides for 10(mu) g DNA sample. The procedures of luminescent method is very simple and low- cost. IT appears applicable to the ultra sensitive detection of low levels of DNA adducts without radioactive isotope.

Paper Details

Date Published: 14 September 2001
PDF: 4 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440151
Show Author Affiliations
Shunqing Xu, Huazhong Univ. of Science and Technology (China)
Xianglin Tan, Huazhong Univ. of Science and Technology (China)
Qunfeng Yao, Huazhong Univ. of Science and Technology (China)
Min He, Huazhong Univ. of Science and Technology (China)
Yikai Zhou, Huazhong Univ. of Science and Technology (China)
Jian Chen, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

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