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Proceedings Paper

Monitoring of binding of aptamer to protein by fluorescent anisotropy
Author(s): Xiaokun Cai; Shunqing Xu; Zhiren Zhang; Zhiwei Liu; Wenhong Lu
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Paper Abstract

The anti-thrombin aptamer can recognize and interact with thrombin in high affinity. This interaction results in the change of the fluorescent anisotorpy in the solution of the aptamer labeled with fluorescence. We developed a new method based on the change of the fluorescent anisotropy intensity resulted from this interaction. The change in polarization was depended on the concentration of thrombin. The linear range of the determination is 0.1-100ng/ml. The detection limit for thrombin is 0.1 ng/ml. This method has been used to determine thrombin in human whole blood. Aptamer that change the fluorescence characteristics of attached fluorophore will be useful in noncompetitive fluorescence polarization assay.

Paper Details

Date Published: 14 September 2001
PDF: 3 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440150
Show Author Affiliations
Xiaokun Cai, Huazhong Univ. of Science and Technology (China)
Shunqing Xu, Huazhong Univ. of Science and Technology (China)
Zhiren Zhang, Huazhong Univ. of Science and Technology (China)
Zhiwei Liu, Huazhong Univ. of Science and Technology (China)
Wenhong Lu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)

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