Share Email Print

Proceedings Paper

Modeling of the orientational and structural dynamics of single-stranded DNA probe molecules immobilized on fused silica and glass surfaces
Author(s): Jiang Zeng; Xiaofeng Wang; Ulrich J. Krull
Format Member Price Non-Member Price
PDF $17.00 $21.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Immobilization of single-stranded DNA onto fused silica and glass surfaces has been widely used for preparation of fiber optic sensors and DNA microarrays. Fundamental investigations of the orientation, motion and hybridization behavior of immobilized ssDNA is important to understand the design sensing devices. Using computational methods to evaluate molecular dynamics w have simulated a solid SiO2-linker-ssDNA system and studied the conformations assumed by such immobilized material under different physical constraints. We have also evaluated the molecular dynamics of fluorescent intercalating dyes that are attached to the ssDNA by tethers of various lengths, with the goal of preparing a label on ssDNA that will transducer hybridization. Periodic boundary conditions were applied to examine packing properties and nearest neighbor interactions of adjacent ssDNA molecules. Molecular dynamics have been performed at room temperature as well as on heated systems. Solvent effects were taken into account and the modeling assumed the presence of an aqueous environment. The results suggest the various conformations and orientations that immobilized ssDNA can assume, and indicate that the orientation is generally far more that is perpendicular to the interface. Exploration of tether lengths indicates the minimum lengths that are required for a fluorescent label to operate efficiently as an intercalating agent.

Paper Details

Date Published: 14 September 2001
PDF: 7 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440128
Show Author Affiliations
Jiang Zeng, Univ. of Toronto at Mississauga (Canada)
Xiaofeng Wang, Univ. of Toronto at Mississauga (Canada)
Ulrich J. Krull, Univ. of Toronto at Mississauga (Canada)

Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)
Yikai Zhou; Shunqing Xu, Editor(s)

© SPIE. Terms of Use
Back to Top