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Proceedings Paper

Photodynamic treatment of lens epithelial cells for cataract surgery
Author(s): Robert W. Lingua; Jean-Marie A. Parel; Gabriel Simon; Kam Li
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Paper Abstract

Photodynamic therapy (PDT) eiiploying Dihematopor*iyrin ethers (DHE) (Photofrin II) at pharmacologic lvels, has been denonstrate3 to kill rabbit lens epithelial cells, in vivo. This in vitro study, reports on the minimal necessary parameters for rabbit lens epithelial cell death. Explants of rabbit lenses were incubated in various concentrations of DHE (1O,, 100, 500, 1000 ug/ml) for 1, 2, or 5 minutes. 30 to 120 Joules/an of collimated 514.5 nm Argon laser light re delivered to the locier concentrations of 10, 50, and 100 ug,'ml DHE treated cells. One hundre1 fifteen explants were treated, in all. Higher concentrations of DHE alone (500 and 1000 ug/ml) were sufficient to induce cellular swelling. Lower concentrations required light for cellular effect. Trypan blue staining revealed cell death at these minimal pa9ieters: DHE 50 ug/ml, incubation 1 minute, 514.5 r Argon light 1.0 Watt/an for 30 sec (30 Joules) . In future studies, these rameters will be tested in vivo, for their ability to eliminate lens epithelial proliferation after cataract surgery.

Paper Details

Date Published: 1 June 1991
PDF: 4 pages
Proc. SPIE 1423, Ophthalmic Technologies, (1 June 1991); doi: 10.1117/12.43962
Show Author Affiliations
Robert W. Lingua, Bascom Palmer Eye Institute/Univ. of Miami and Loma Linda Un (United States)
Jean-Marie A. Parel, Bascom Palmer Eye Institute/Univ. of Miami and Loma Linda Un (United States)
Gabriel Simon, Bascom Palmer Eye Institute/Univ. of Miami and Loma Linda Un (France)
Kam Li, Bascom Palmer Eye Institute/Univ. of Miami and Loma Linda Un (United States)


Published in SPIE Proceedings Vol. 1423:
Ophthalmic Technologies
Carmen A. Puliafito, Editor(s)

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