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Proceedings Paper

Novel photobleaching model in living cells
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Paper Abstract

A novel photobleaching model was proposed, in which a D-P photobleaching mechanism was developed. By combining with the conventional D-D and D-O photobleaching mechanisms, the dependence of photobleaching rate on the excitation power is well illustrated. The validity of this model was demonstrated with GFP photobleaching experiments in cases of one-photon excitation (1PE) and two-photon excitation (2PE) respectively. Previously inexplicable experimental results published in literature were also illustrated with this new model.

Paper Details

Date Published: 22 August 2001
PDF: 7 pages
Proc. SPIE 4427, Imaging of Tissue Structure and Function, (22 August 2001); doi: 10.1117/12.438419
Show Author Affiliations
Tongsheng Chen, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)
Wei Zhou, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 4427:
Imaging of Tissue Structure and Function

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