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Proceedings Paper

Optical properties of human blood as a function of temperature
Author(s): Lise Lyngsnes Randeberg; Anre Johan Daae Hagen; Lars Othar Svaasand
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Paper Abstract

Knowledge of optical and thermal properties of human tissue is essential for optimization of laser therapy and optical diagnostics. Published data are in general incomplete, and there is very limited information on the temperature dependence of optical properties. The temperature dependence of hemoglobin is important since it is the target chromophore for laser treatment of e. g. port-wine stains. The present study emphasizes the temperature dependence of the hemoglobin absorption. Erythrocyte concentrate was heated in a water bath, hemolyzed with distilled water and centrifuged before absorption spectra were collected. Measurements were performed in the range of 50-80 °C. Penetration depth measurements of the heated erythrocyte concentrate were done at 488 nm, 585 nm, 590 nm and 630 nm. For these measurements the erythrocytes were mixed with a highly scattering material (whole milk). The hemoglobin absorbance demonstrates a characteristic change during heating. This change is partly due to oxidative reactions with formation of met-hemoglobin, and also by protein denaturation. The change in the absorption coeÆcient due to met-hemoglobin has a maximum at approximately 72 °C, where the increase was measured up to 200 % at 630 nm wavelength.

Paper Details

Date Published: 17 June 2002
PDF: 9 pages
Proc. SPIE 4609, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XII, (17 June 2002); doi: 10.1117/12.437283
Show Author Affiliations
Lise Lyngsnes Randeberg
Anre Johan Daae Hagen
Lars Othar Svaasand

Published in SPIE Proceedings Vol. 4609:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XII
Lawrence S. Bass M.D.; Eugene A. Trowers M.D.; Kenneth Eugene Bartels D.V.M.; Udayan K. Shah M.D.; David S. Robinson M.D.; Lawrence S. Bass M.D.; Kenton W. Gregory M.D.; Kenneth Eugene Bartels D.V.M.; Lawrence S. Bass M.D.; Brian Jet-Fei Wong M.D.; Hans-Dieter Reidenbach; Lloyd P. Tate V.D.M.; Nikiforos Kollias; Abraham Katzir; Timothy A. Woodward M.D.; Werner T.W. de Riese; George M. Peavy D.V.M.; Werner T.W. de Riese; Kenton W. Gregory M.D.; Michael D. Lucroy D.V.M.; Abraham Katzir; Nikiforos Kollias; Michael D. Lucroy D.V.M.; Reza S. Malek M.D.; George M. Peavy; Hans-Dieter Reidenbach; David S. Robinson M.D.; Udayan K. Shah M.D.; Lloyd P. Tate V.D.M.; Eugene A. Trowers M.D.; Brian Jet-Fei Wong M.D.; Timothy A. Woodward M.D., Editor(s)

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