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Proceedings Paper

Detection of motile micro-organisms in biological samples by means of a fully automated image processing system
Author(s): Elvio Alanis; Graciela Romero; Liliana Alvarez; Carlos C. Martinez; Daniel Hoyos; Miguel A. Basombrio
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Paper Abstract

A fully automated image processing system for detection of motile microorganism is biological samples is presented. The system is specifically calibrated for determining the concentration of Trypanosoma Cruzi parasites in blood samples of mice infected with Chagas disease. The method can be adapted for use in other biological samples. A thin layer of blood infected by T. cruzi parasites is examined in a common microscope in which the images of the vision field are taken by a CCD camera and temporarily stored in the computer memory. In a typical field, a few motile parasites are observable surrounded by blood red cells. The parasites have low contrast. Thus, they are difficult to detect visually but their great motility betrays their presence by the movement of the nearest neighbor red cells. Several consecutive images of the same field are taken, decorrelated with each other where parasites are present, and digitally processed in order to measure the number of parasites present in the field. Several fields are sequentially processed in the same fashion, displacing the sample by means of step motors driven by the computer. A direct advantage of this system is that its results are more reliable and the process is less time consuming than the current subjective evaluations made visually by technicians.

Paper Details

Date Published: 14 August 2001
PDF: 4 pages
Proc. SPIE 4419, 4th Iberoamerican Meeting on Optics and 7th Latin American Meeting on Optics, Lasers, and Their Applications, (14 August 2001); doi: 10.1117/12.437155
Show Author Affiliations
Elvio Alanis, Univ. Nacional de Salta (Argentina)
Graciela Romero, Univ. Nacional de Salta (Argentina)
Liliana Alvarez, Univ. Nacional de Salta (Argentina)
Carlos C. Martinez, Univ. Nacional de Salta (Argentina)
Daniel Hoyos, Univ. Nacional de Salta (Argentina)
Miguel A. Basombrio, Univ. Nacional de Salta (Argentina)

Published in SPIE Proceedings Vol. 4419:
4th Iberoamerican Meeting on Optics and 7th Latin American Meeting on Optics, Lasers, and Their Applications
Vera L. Brudny; Silvia A. Ledesma; Mario C. Marconi, Editor(s)

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