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Proceedings Paper

Photothermal stimulation of chondrocyte proliferation in ex-vivo cartilage grafts
Author(s): Mai Thy Truong; David Gardener; Nidhi S. Pandoh; Brian Jet-Fei Wong
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Paper Abstract

In vivo, laser radiation has been shown to stimulate cartilage repair and proliferation, which is of clinical relevance as light can be delivered using minimally invasive techniques. However, dosimetry and temperature dependence of this phenomenon have neither been determined nor have these findings been conclusively demonstrated ex vivo. In this study, we detected the presence of proliferating chondrocytes in intact laser irradiated rabbit septal cartilage using a novel whole mount Bromodeoxyuridine (BrdU) assay, and determined the dependence of this phenomenon on laser dosimetry. Cartilage specimens were irradiated with light from an Nd:YAG laser (λ= 1.32 μm, 3-16 sec, 10-45 W/cm2) and placed in tissue culture with BrdU for 7-9 days. BrdU (a thymidine analogue) is incorporated into DNA during replication. Specimens were then fixed and treated with an enzyme-linked double antibody system providing a color change to indicate the presence of BrdU in dividing cells. The samples were analyzed in whole mount and with conventional histology. Proliferation was clearly identified for laser exposures greater than 6 seconds at (25 W/cm2), and was observed only on the periphery of the laser spot. This study clearly demonstrates that laser heating of ex vivo cartilage tissue results in chondrocyte proliferation. Inasmuch as this phenomenon was observed in tissue culture, the non-specific cellular and humoral responses present an intact organism were eliminated. Cell division likely results form either changes in the fine structure of the tissue matrix or direct stimulation of chondrocyte metabolism.

Paper Details

Date Published: 9 July 2001
PDF: 7 pages
Proc. SPIE 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, (9 July 2001); doi: 10.1117/12.434741
Show Author Affiliations
Mai Thy Truong, Beckman Laser Institute and Medical Clinic (United States)
David Gardener, Univ. of California/Irvine (United States)
Nidhi S. Pandoh, Beckman Laser Institute and Medical Clinic (United States)
Brian Jet-Fei Wong, Beckman Laser Institute and Medical Clinic and Univ. of California/Irvine (United States)

Published in SPIE Proceedings Vol. 4257:
Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical
Donald Dean Duncan; Donald Dean Duncan; Steven L. Jacques; Peter C. Johnson; Peter C. Johnson, Editor(s)

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