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Proceedings Paper

Frequency-domain fluorescent diffusion tomography of turbid media and in-vivo tissues
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Paper Abstract

The reconstruction of fluorescence lifetime distributions in heterogeneous turbid media and tumor-bearing animals are experimentally demonstrated by frequency-domain measurements. A set of coupled diffusion equations are used to describe the propagation of excitation and fluorescent emission light in multiply scattering media. A finite element based reconstruction algorithm combined with Marquardt and Tikhonov regularization methods are used to obtain the fluorescence images. The experimental set-up is an automatic multi-channel frequency-domain system. 16 sources and 16 detectors are used. Experiments are performed using indocyanine green (ICG) and 3,3'-diethylthiatricarbocyanine iodide (DTTCI) in tissue-like phantoms of both single- and multi-target configurations with considerations of perfect and imperfect uptake of fluorescence dyes in the scattering media. ICG are used in tumor-bearing animal studies. Our results show that the fluorescence lifetime image of the heterogeneities within a circular surrounding medium and in-vivo tissue can be reconstructed successfully.

Paper Details

Date Published: 29 June 2001
PDF: 9 pages
Proc. SPIE 4250, Optical Tomography and Spectroscopy of Tissue IV, (29 June 2001); doi: 10.1117/12.434529
Show Author Affiliations
Ye Yang, Clemson Univ. (United States)
Nicusor Iftimia, Clemson Univ. (United States)
Yong Xu, Clemson Univ. (United States)
Huabei Jiang, Clemson Univ. (United States)

Published in SPIE Proceedings Vol. 4250:
Optical Tomography and Spectroscopy of Tissue IV
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva Marie Sevick-Muraca, Editor(s)

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