Share Email Print
cover

Proceedings Paper

In-vitro correlation between reduced scattering coefficient and hemoglobin concentration of human blood determined by near-infrared spectroscopy
Author(s): Lelia Adelina Paunescu; Antonios Michalos; Jee Hyun Choi; Ursula Wolf; Martin Wolf; Enrico Gratton
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We study the correlation between (mu) s' and THC obtained in vitro, in a highly scattering medium containing human blood. We used a frequency domain near infrared spectrometer (modulation frequency: 110 MHz, wavelengths: 758 and 830 nm) to measure in real time (acquisition time: 0.64 s) (mu) s' and THC. We used Liposyn suspension and red blood cells in saline buffer solution. After a couple of minutes of baseline acquisition, several consecutive increments of 3-5 ml blood were added to the solution yielding THC equals 15-100 (mu) M and (mu) a equals 0.03-0.3 1/cm. At the last amount of blood added, increments of glucose in the range of 0.5-20 g/L were added. For each step of blood and glucose added, data were acquired for a couple of minutes. This was repeated 6 times. Average of data was calculated for both (mu) s' and THC for each of the red blood cells and glucose increments added. We found a high correlation between (mu) s' and THC (0.018 X THC + 4.51, R2 equals 0.98 at 758 nm and 0.012 X THC + 4.86, R2 equals 0.97 at 830 nm). We studied the effect of glucose on (mu) s' and we found a high correlation between the glucose added to the suspension and the decrease in (mu) s' for the case of high glucose concentrations. The slope of this correlation is -0.011 at both wavelengths and the correlation factors were R2 X 0.96 at 830 nm and R2 equals 0.91 at 758 nm (case shown). The effect of glucose was less significant at 830 nm than at 758 nm in general. This work is a proof of principle for detection of (mu) s' changes with glucose. This approach also establishes limits for glucose detection in physiological conditions.

Paper Details

Date Published: 29 June 2001
PDF: 8 pages
Proc. SPIE 4250, Optical Tomography and Spectroscopy of Tissue IV, (29 June 2001); doi: 10.1117/12.434505
Show Author Affiliations
Lelia Adelina Paunescu, Univ. of Illinois/Urbana-Champaign (United States)
Antonios Michalos, Univ. of Illinois/Urbana-Champaign (United States)
Jee Hyun Choi, Univ. of Illinois/Urbana-Champaign (United States)
Ursula Wolf, Univ. of Illinois/Urbana-Champaign (United States)
Martin Wolf, Univ. of Illinois/Urbana-Champaign (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 4250:
Optical Tomography and Spectroscopy of Tissue IV
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva Marie Sevick-Muraca, Editor(s)

© SPIE. Terms of Use
Back to Top