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Proceedings Paper

Scanning acoustic microscopy reveals distinct motility domains in living cells
Author(s): Ilonka Karl; Juergen Bereiter-Hahn
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Paper Abstract

In the middle of the 90s, the image processing and analysing method subtraction scanning acoustic microscopy (SubSAM) was developed to display and quantify cell surface motility. Since then, the approach of this method supported already existing models and provided new insights into the regulation of cell surface motility. The investigations with SubSAM revealed that cell surface motility is modulated by tension and is regulated by known F-actin reorganising signal transduction pathways via the small GTP-binding protein Rac and protein kinase C. Beyond that, the in vitro cell surface motility as revealed by SubSAM corelates with cell transformation respectively the invasive or metastatic potential. The enhancement of cell surface motility is always accompanied by characteristic actin reorganisation: loss of stress fibers and the formation of cortical F-actin knots or larger aggregates. From these observations a model has been developed for the regulation of cell surface motility and an addtitional mechanism for cell surface deformation based on myosin-dependent F-actin aggregation is proposed.

Paper Details

Date Published: 24 July 2001
PDF: 7 pages
Proc. SPIE 4335, Advanced Nondestructive Evaluation for Structural and Biological Health Monitoring, (24 July 2001); doi: 10.1117/12.434181
Show Author Affiliations
Ilonka Karl, J.W. Goethe Univ. (Germany)
Juergen Bereiter-Hahn, J.W. Goethe Univ. (Germany)

Published in SPIE Proceedings Vol. 4335:
Advanced Nondestructive Evaluation for Structural and Biological Health Monitoring
Tribikram Kundu, Editor(s)

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