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Proceedings Paper

Physicist's simple access to protein structures: the computer program WHAT IF
Author(s): Brigitte Altenberg-Greulich; Stephan G. Zech; Dietmar Stehlik; Gert Vriend
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Paper Abstract

We describe the computer program WHAT IF and its application to two physical examples. For the DNA binding protein, OCT-1 (pou domain) the location of amino acids with a sidechain amino group is shown. Such knowledge is required when staining this molecule with a fluorescence dye, which binds chemically to the amino terminus as well as amino groups in sidechains. The program shows that most sidechain amino groups are protected when DNA is bound to OCT-1, allowing selective staining of the amino terminal NH2 group. A protein stained this way can be used in fluorescence spectroscopic studies on function aspects of OCT-1.

Paper Details

Date Published: 29 June 2001
PDF: 6 pages
Proc. SPIE 4430, ROMOPTO 2000: Sixth Conference on Optics, (29 June 2001); doi: 10.1117/12.432914
Show Author Affiliations
Brigitte Altenberg-Greulich, European Molecular Biology Lab. (Germany)
Stephan G. Zech, Freie Univ. Berlin (Germany)
Dietmar Stehlik, Freie Univ. Berlin (Germany)
Gert Vriend, CMBI-KUN (Netherlands)


Published in SPIE Proceedings Vol. 4430:
ROMOPTO 2000: Sixth Conference on Optics

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