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Proceedings Paper

New method to measure the contraction speed of corneal collagen under laser irradiation
Author(s): Traian Dascalu; G. Cruz de Leon; M. Consolacion Martinez Saldana; C. J. Ventura Juarez
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Paper Abstract

Laser thermokeratoplasty (LTK) is a very promising method to correct hyperopia and astigmatism. Thermally induced shrinkage of corneal collagen in corneal periphery with IR lasers produce an increasing of central corneal curvature. In order to increase the accuracy of changes in corneal curvature we develop the method to find out the dependence of the collagen's total contraction and contraction speed under laser irradiation. The experiments were performed on porcine eyes, in vitro, by using the fluorescence photobleaching (FP) method and CTH:YAG laser Nd:YAG laser. The collagen fibril was marked with fluorescent collagen antibody. CTH:YAG was used to produce fibril shrinkage and simultaneously an argon laser pulse produce a bleaching pattern on fluorescent marked area. By using the fluorescence microscope the signal detected by a photomultiplier is analyzed and the speed of fluorescent marker and contraction length are detected.

Paper Details

Date Published: 29 June 2001
PDF: 7 pages
Proc. SPIE 4430, ROMOPTO 2000: Sixth Conference on Optics, (29 June 2001); doi: 10.1117/12.432904
Show Author Affiliations
Traian Dascalu, Unidad Aguascalientes (Mexico)
G. Cruz de Leon, Unidad Aguascalientes (Mexico)
M. Consolacion Martinez Saldana, Free Univ. of Aguascalientes (Mexico)
C. J. Ventura Juarez, Free Univ. of Aguascalientes (Mexico)


Published in SPIE Proceedings Vol. 4430:
ROMOPTO 2000: Sixth Conference on Optics

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