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Proceedings Paper

Spatial imaging of cooperative systems in capillaries (Hb, HbO2) and cells (Redox states and changes of subcellular functional structures) in perfused liver
Author(s): Markus Boehnert; Robert Rauh; Manfred D. Kessler
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Paper Abstract

Light scattering in living tissues is mainly caused by subcellular particles like mitochondria. The size of mitochondria changes according to differences in the functional status. Therefore light scattering should be a useful technique for monitoring the functional state in tissues. We investigated functional parameters in our model of the isolated perfused rat liver. For the measurements of light scattering we used the EMPHO SSK Oxyscan. Backscattered light from tissue is shown in 3D images. We found an interesting relation between structures of the liver and the patterns of the relating 3D images. In addition, our underlying spectra show the redox state of cytochromes. This new method of tissue imaging should give the opportunity of new insights into liver function.

Paper Details

Date Published: 4 May 2001
PDF: 8 pages
Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); doi: 10.1117/12.431569
Show Author Affiliations
Markus Boehnert, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)
Robert Rauh, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)
Manfred D. Kessler, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)


Published in SPIE Proceedings Vol. 4241:
Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II

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