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Proceedings Paper

Spectral measurements of dc potentials in perfused rat liver by the use of Merocyanine-540
Author(s): Christine Mahlke; Markus Boehnert; Robert Rauh; Manfred D. Kessler
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Paper Abstract

In organs we can find signal changes between vascular and parenchymal cells. We started to combine spectral measurements by the use of merocyanine in isolated perfused rat liver to analyze the alterations of dc-potentials. There have been first experiments with Merocyanine (M-540) in the seventies with stained axons and hearts to measure optical action potentials (Morad, Salama, et al, 1978). It is reported that the increase of fluorescence response is connected to a depolarization and its decrease to a repolarization. Tissue imaging after staining with this dye should be ideal for a long term interpretation of dc-potential alterations as an analysis of the electrical coupling in different cell types under various experimental conditions like anoxia or temperature changes.

Paper Details

Date Published: 4 May 2001
PDF: 9 pages
Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); doi: 10.1117/12.431558
Show Author Affiliations
Christine Mahlke, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)
Markus Boehnert, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)
Robert Rauh, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)
Manfred D. Kessler, Institute of Physiology and Cardiology/Univ. of Erlangen-Nuremberg (Germany)


Published in SPIE Proceedings Vol. 4241:
Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II

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