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Proceedings Paper

Fluorescence imaging of endothelial cellular responses in the intact lung microvasculature
Author(s): Wolfgang Michael Kuebler; Corinna Brueckl; Andreas Kuehnl; Xiaoyou Ying; Jahar Bhattacharya
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Paper Abstract

Endothelial cells outline the blood vessel wall and contribute pivotally to the regulation of numerous microvascular characteristics such as permeability, vascular tone, vasomotion, or cell-cell interactions. These functions are mediated by second messenger responses and intracellular signal transduction cascades within the endothelial cells. Imaging of endothelial cellular and subcellular responses has yet been limited to studies in isolated or cultured cells, which cannot reflect dynamic physiologic or pathologic cellular interactions in the intact microcirculation. Combining micropuncture and microcatheter approaches with in vitro fluorescence imaging techniques, we have developed methods for imaging endothelial cellular responses of intact pulmonary microvessels in situ.

Paper Details

Date Published: 4 May 2001
PDF: 10 pages
Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); doi: 10.1117/12.431551
Show Author Affiliations
Wolfgang Michael Kuebler, Univ. of Munich and Columbia Univ. (Germany)
Corinna Brueckl, Univ. of Munich (Germany)
Andreas Kuehnl, Univ. of Munich (Germany)
Xiaoyou Ying, Columbia Univ. (United States)
Jahar Bhattacharya, Columbia Univ. (United States)

Published in SPIE Proceedings Vol. 4241:
Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II
Valery V. Tuchin, Editor(s)

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