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Proceedings Paper

Single-molecule probes
Author(s): Laurie S. Goldner; Kenneth D. Weston; William F. Heinz; Jeeseong Hwang; Eric S. DeJong; John P. Marino
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Paper Abstract

The technology to rapidly manipulate and screen individual molecules lies at the frontier of measurement science, with impacts in bio- and nano-technology. Fundamental biological and chemical processes can now be probed with unprecedented detail, one molecule at a time. These single molecule probes are most often fluorescent dye molecules embedded in a material or attached to a target molecule, such as a protein or nucleic acid, whose behavior us under study. The fluorescence from a single dye molecule can be detected, its spectrum and lifetime measured and its absorption or emission dipole calculated. From this information, the rotational and translational dynamics of the fluorophore can be calculated, as can details of its photophysics. To the extent that these properties reflect the properties of the target molecule, we can use these fluorescent tags to probe the dynamics and structure of the target. In this work we discuss the dependence of the physical and photophysical dynamics of fluorescent molecules on their local environment, and we use confocal microscopy to study single molecules in thin films, on surfaces, and in various liquid and gaseous environments.

Paper Details

Date Published: 18 June 2001
PDF: 11 pages
Proc. SPIE 4450, Harnessing Light: Optical Science and Metrology at NIST, (18 June 2001); doi: 10.1117/12.431255
Show Author Affiliations
Laurie S. Goldner, National Institute of Standards and Technology (United States)
Kenneth D. Weston, National Institute of Standards and Technology (Germany)
William F. Heinz, National Institute of Standards and Technology (United States)
Jeeseong Hwang, National Institute of Standards and Technology (United States)
Eric S. DeJong, National Institute of Standards and Technology (United States)
John P. Marino, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 4450:
Harnessing Light: Optical Science and Metrology at NIST
Carmina Londono, Editor(s)

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