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Proceedings Paper

Detection of DNA and P-450s on silver colloidal nanoparticles by surface-enhanced resonance Raman scattering (SERRS)
Author(s): W. Ewen Smith; Ewan Polwart; Clare McLaughlin; Benjamin J. Mallinder; Susan J. Smith; Duncan Graham
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Paper Abstract

Surface-enhanced resonance Raman scattering (SERRS) is a very sensitive and selective detection method that can be used for the analysis of both DNA and P-450s. A number of factors have limited the broader application of the technique. These limitations are described and addressed. An approach to reduce the problems associated with variation of the silver colloids used to provide surface enhancement and chemical methodologies that ensure surface adsorption are presented. A practical approach was used to investigate the nature of the effect. This approach has highlighted the importance of resonance enhancement for ultimate sensitivity. Two approaches to achieve successful detection of DNA using SERRS are described, and, using these two approaches, the possibility of multiplexing is also demonstrated. The analysis of proteins by SERRS is discussed and P-450 is presented as a specific example of the information that may be gained from SERRS of proteins.

Paper Details

Date Published: 18 June 2001
PDF: 7 pages
Proc. SPIE 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications, (18 June 2001); doi: 10.1117/12.430763
Show Author Affiliations
W. Ewen Smith, Univ. of Strathclyde (United Kingdom)
Ewan Polwart, Univ. of Strathclyde (United Kingdom)
Clare McLaughlin, Univ. of Strathclyde (United Kingdom)
Benjamin J. Mallinder, Univ. of Strathclyde (United Kingdom)
Susan J. Smith, Univ. of Strathclyde (United Kingdom)
Duncan Graham, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 4258:
Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications
Catherine J. Murphy, Editor(s)

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