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Proceedings Paper

Fluorescence method for monitoring of glucose in interstitial fluids
Author(s): Maxim V. Loshchenov; Alexandr S. Parfenov; Gennady L. Kisselev; Alexander A. Stratonnikov; Katerina Ershova; Rudolf W. Steiner
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Paper Abstract

Development of minimal invasive diagnostics methods is of great importance in today's healthcare. The present work deals with the measurements of glucose concentration in small drops, its volume being in the range of 1 (mu) L and its glucose concentration being in the range of 1-10 mM. The aim of the work was to develop robust quantitative method of glucose measurement in small volumes of interstitial fluid obtained after laser shot or by other low invasive procedures. The commercially available Amlex Red glucose assay kit was used in the present work. The glucose is detected by enzyme-coupling reactions resulting in formation of fluorescence dye resorufin. The diagnostic paper was used as a reaction medium. The fluorescence of dye in diagnostic paper was measured with the use of fiber optics spectrometer. It was shown that dye fluorescence correlates fairly well with glucose concentration. The method may be modified to involve cholesterol concentration measurement as well.

Paper Details

Date Published: 13 June 2001
PDF: 6 pages
Proc. SPIE 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring, (13 June 2001); doi: 10.1117/12.429344
Show Author Affiliations
Maxim V. Loshchenov, General Physics Institute (Russia)
Alexandr S. Parfenov, Research Institute of Physical Chemical Medicine (United States)
Gennady L. Kisselev, General Physics Institute (Russia)
Alexander A. Stratonnikov, General Physics Institute (Russia)
Katerina Ershova, General Physics Institute (Russia)
Rudolf W. Steiner, Ulm Univ. (Germany)

Published in SPIE Proceedings Vol. 4263:
Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring
Alexander V. Priezzhev; Gerard L. Cote, Editor(s)

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