Share Email Print
cover

Proceedings Paper

Detection of apoptosis by optical coherence tomography (OCT)
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Apoptosis is the effector of regulated cell death and plays a role in many physiologic and pathologic processes. It is characterized by a highly regulated condensation and fragmentation of the cell nucleus, a large scatterer, and breakup of the entire cell into vesicles, (apoptotic bodies) containing cell organelles and fragments of the nucleus. A two-fold increase in attenuation coefficient ((mu) ) is observed in cell culture after chemical induction of apoptosis. An identical increase in scattering is observed in a tissue culture of porcine carotid artery, in which apoptosis is induced by balloon dilation. These observations are theoretically supported by calculations based on MIE theory. The preliminary results of this study indicate that the apoptotic process may be detected using OCT due to an increase in scattering by the typical disintegration of cellular material. The described increase in scattering may also be detected by other optical techniques.

Paper Details

Date Published: 23 May 2001
PDF: 5 pages
Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); doi: 10.1117/12.427888
Show Author Affiliations
Freek J. van der Meer, Academic Medical Ctr./Univ. of Amsterdam (Netherlands)
Dirk J. Faber, Academic Medical Ctr./Univ. of Amsterdam (Netherlands)
Maurice C.G. Aalders, Academic Medical Ctr./Univ. of Amsterdam (Netherlands)
Jop Perree, Academic Medical Ctr./Univ. of Amsterdam (Netherlands)
Ton G. J. M. van Leeuwen, Academic Medical Ctr./Univ. of Amsterdam (Netherlands)


Published in SPIE Proceedings Vol. 4251:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

© SPIE. Terms of Use
Back to Top