Share Email Print
cover

Proceedings Paper

In-vitro monitoring of redox state of cytochrome oxidase in bone by optical coherence quantitation based on low-coherence interferometry
Author(s): Xiangqun Xu; Ruikang K. Wang; Alicia J. El Haj
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We present optical coherence quantitation technique to monitor the redox state of mitochondria enzyme Cytochrome oxidase (CytOx) in bone tissue by the use of optical coherence tomography (OCT) system. Superluminescent diode (SLD) with its peak emission wavelength ((lambda) = 820nm) on the absorption band of oxidized form of CytOx was used in the experiments. The reflectance returning from the liquid phantoms (naphthol green B with intralipid) and bone tissue specimens (periosteum of calvaria from newborn rats) as a function of penetration depth was used to quantify the absorption changes of the sample. Absorption coefficients of naphthol green B were accurately quantified by the linear relationship between attenuation coefficients from the slopes of the reflected signals and naphthol green B concentration. The results show that the attenuation coefficient decreases in periosteums as CytOx is reduced by sodium dithionite, demonstrating the feasibility of this method to quantify the redox state of tissues studied. A 70% +/- 7% (n=4) reduction of attenuation coefficients in periosteums was clearly observed with redox change of CytOx after 5 min reduction. In addition, the results demonstrate that the OCT system is also capable of imaging the calvaria tomographically with a resolution at 9 microns, which could only be previously obtained by the conventional excisional biopsy.

Paper Details

Date Published: 23 May 2001
PDF: 6 pages
Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); doi: 10.1117/12.427880
Show Author Affiliations
Xiangqun Xu, Keele Univ. School of Postgraduate Medicine (United Kingdom)
Ruikang K. Wang, Keele Univ. School of Postgraduate Medicine (United Kingdom)
Alicia J. El Haj, Keele Univ. School of Postgraduate Medicine (United Kingdom)


Published in SPIE Proceedings Vol. 4251:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

© SPIE. Terms of Use
Back to Top