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Proceedings Paper

Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibodies in blood sera of domestic cats
Author(s): Janaina Duarte; Marcos T. T. Pacheco; Landulfo Silveira; Rosangela Zacarias Machado; Rodrigo A.B. Lopes Martins; Renato Amaro Zangaro; Antonio G. J. Balbin Villaverde
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Paper Abstract

Near-infrared (NIR) Raman spectroscopy has been studied for the last years for many biomedical applications. It is a powerful tool for biological materials analysis. Toxoplasmosis is an important zoonosis in public health, cats being the principal responsible for the transmission of the disease in Brazil. The objective of this work is to investigate a new method of diagnosis of this disease. NIR Raman spectroscopy was used to detect anti Toxoplasma gondii antibodies in blood sera from domestic cats, without sample preparation. In all, six blood serum samples were used for this study. A previous serological test was done by the Indirect Immunoenzymatic Assay (ELISA) to permit a comparative study between both techniques and it showed that three serum samples were positive and the other three were negative to toxoplasmosis. Raman spectra were taken for all the samples and analyzed by using the principal components analysis (PCA). A diagnosis parameter was defined from the analysis of the second and third principal components of the Raman spectra. It was found that this parameter can detect the infection level of the animal. The results have indicated that NIR Raman spectroscopy, associated to the PCA can be a promising technique for serological analysis, such as toxoplasmosis, allowing a fast and sensitive method of diagnosis.

Paper Details

Date Published: 21 May 2001
PDF: 8 pages
Proc. SPIE 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI, (21 May 2001); doi: 10.1117/12.427767
Show Author Affiliations
Janaina Duarte, Univ. do Vale do Paraiba (Brazil)
Marcos T. T. Pacheco, Univ. do Vale do Paraiba (Brazil)
Landulfo Silveira, Univ. do Vale do Paraiba (Brazil)
Rosangela Zacarias Machado, Univ. Estadual Paulista (Brazil)
Rodrigo A.B. Lopes Martins, Univ. do Vale do Paraiba (Brazil)
Renato Amaro Zangaro, Univ. do Vale do Paraiba (Brazil)
Antonio G. J. Balbin Villaverde, Univ. do Vale do Paraiba (Brazil)

Published in SPIE Proceedings Vol. 4244:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI
Lawrence S. Bass; David S. Robinson; Eugene A. Trowers; Kenton W. Gregory; Lou Reinisch; Kenneth Eugene Bartels; R. Rox Anderson; Reza S. Malek; Kenneth Eugene Bartels; Lloyd P. Tate; Abraham Katzir; C. Gaelyn Garrett; Timothy A. Woodward; Hans-Dieter Reidenbach; George M. Peavy; Lawrence S. Bass; C. Gaelyn Garrett; Michael D. Lucroy; Kenton W. Gregory; Abraham Katzir; Nikiforos Kollias; Michael D. Lucroy; Reza S. Malek; J. Stuart Nelson; George M. Peavy; Hans-Dieter Reidenbach; Lou Reinisch; David S. Robinson; Lloyd P. Tate; Eugene A. Trowers; Timothy A. Woodward, Editor(s)

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