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Proceedings Paper

Application of computerized interference microscope for monitoring oscillations of dry cell weight and morphology of living cells
Author(s): Gennady G. Levin; Theodor V. Bulygin; Eugene Kalinin; Gennady N. Vishnyakov
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Paper Abstract

Interference microscopy technique allows implementing the quantitative analysis of a living cell, for example, to measure dry cell weight and cell density spatial distribution. The authors have created the microscope for quantitative analysis of living cells and observation of the dynamics of cell vital activity. Computer-aided processing of interferograms using phase shift technique, allows carrying out continuous (over 12 hours and more) monitoring of quantitative parameters of living cells with time interval between measurements less than one minute. RMS error for dry weight of non-living fixed test object using this microscope is about 1%. Numerous experiments on living lymphocytes and erythrocytes resulted in detecting the oscillations of dry weight. Spectral analysis of obtained time series has allowed allocating harmonics with the period from 2 hours to 15 minutes. Morphometric measurements of the same cells and cross correlation analysis of these data with oscillations of a dry mass were carried out also.

Paper Details

Date Published: 10 May 2001
PDF: 6 pages
Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); doi: 10.1117/12.426766
Show Author Affiliations
Gennady G. Levin, All-Russian Research Institute for Optophysical Measurements (Russia)
Theodor V. Bulygin, All-Russian Research Institute for Optophysical Measurements (Russia)
Eugene Kalinin, All-Russian Research Institute for Optophysical Measurements (Russia)
Gennady N. Vishnyakov, All-Russian Research Institute for Optophysical Measurements (Russia)


Published in SPIE Proceedings Vol. 4260:
Optical Diagnostics of Living Cells IV
Daniel L. Farkas; Robert C. Leif; Robert C. Leif, Editor(s)

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