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Proceedings Paper

Novel high-sensitivity fluorescence polarization reader
Author(s): Clifford C. Hoyt; Richard M. Levenson; Peter Banks
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Paper Abstract

We have developed a new fluorescence polarization (FP) reader suitable for high-throughput screening (HST) and ultra-HTS whose assay-performance and sample-throughput are both considerably improved over present state-of-the-art instrumentation. The SymmetryTM reader possesses a number of features that differ from conventional HTS FP readers. These include: laser-based excitation, liquid crystal polarization optics that rapidly and accurately measure polarization states; and CCD detectors to capture emission from multiple wells. We show that the performance in assays relevant to the drug discovery process, such as G- protein coupled receptor-based assays, is significantly enhanced due to a dramatic improvement in precision. Furthermore, the CCD-detection system used can substantially improve sample throughput compared to sequential readers while maintaining high performance.

Paper Details

Date Published: 22 May 2001
PDF: 5 pages
Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); doi: 10.1117/12.426755
Show Author Affiliations
Clifford C. Hoyt, CRI, Inc. (United States)
Richard M. Levenson, CRI, Inc. (United States)
Peter Banks, PerkinElmer Life Sciences, Inc. (Canada)

Published in SPIE Proceedings Vol. 4255:
Clinical Diagnostic Systems
Gerald E. Cohn, Editor(s)

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