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Proceedings Paper

Application of diode laser/fiber optic colorimetric spectrometer to the determination of cholesterol
Author(s): SungHo Kim; Shin-Young Yun; Jeong-Hwa Lee; Hyo-Young Lee; Hae-Seon Nam
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Paper Abstract

A simple and inexpensive colorimetric spectrometer for determining total cholesterol has been developed, comprising a diode laser as the light source, optical fibers for the light guide and a photodiode as the detector. In order to generate the color change, enzymatic reaction of cholesterol oxidase-peroxidase method was used. The stability and performance of the new system was investigated by obtaining the calibration curve for standard cholesterol solutions. The total cholesterol in human serum was also measured by the analyzer and compared with the value obtained by a conventional chemistry analyzer. The results showed that the developed spectrometer was useful for the determination of cholesterol levels.

Paper Details

Date Published: 22 May 2001
PDF: 6 pages
Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); doi: 10.1117/12.426744
Show Author Affiliations
SungHo Kim, SoonChunHyang Univ. (South Korea)
Shin-Young Yun, SoonChunHyang Univ. (South Korea)
Jeong-Hwa Lee, SoonChunHyang Univ. (South Korea)
Hyo-Young Lee, SoonChunHyang Univ. (South Korea)
Hae-Seon Nam, SoonChunHyang Univ. College of Medicine (South Korea)

Published in SPIE Proceedings Vol. 4255:
Clinical Diagnostic Systems
Gerald E. Cohn, Editor(s)

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