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Proceedings Paper

Array biosensor for simultaneous detection of multiple analytes
Author(s): Frances S. Ligler; Joel P. Golden; Chris A. Rowe-Taitt; James P. Dodson
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Paper Abstract

The array biosensor has been developed for simultaneous analysis of multiple samples for multiple analytes. A patterned array of capture antibodies is immobilized on the surface of a planar waveguide and a sandwich immunoassay conducted using a cocktail of fluorescent tracer antibodies. Upon excitation of the fluorescent label using a 635 nm diode laser, a CCD camera detects the pattern of fluorescent antigen:antibody complexes on the sensor surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. The assays are fast, sensitive, and specific.

Paper Details

Date Published: 10 May 2001
PDF: 5 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426740
Show Author Affiliations
Frances S. Ligler, Naval Research Lab. (United States)
Joel P. Golden, Naval Research Lab. (United States)
Chris A. Rowe-Taitt, Naval Research Lab. (United States)
James P. Dodson, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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