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Proceedings Paper

Time-resolved fluorescence spectroscopy of dopamine in single cells
Author(s): Michiaki Mabuchi; Junichi Shimada; Koichi Okamoto; Yoichi Kawakami; Shigeo Fujita; Kazumi Matsushige
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Paper Abstract

Dopamine hydrochloric acid salt in aqueous solution was excited at 266 nm Al2O3:Ti laser and the sufficient fluorescence emission peaking at 330 nm was detected with a streak camera. The fluorescence decay curve was fitted by 1- exponential functions, with the lifetime of approximately 0.80 ns. The influence of deep-UV laser excitation on cells is also discussed for the direct observation of dopamine in the living cells. In addition, it is needed to detect the dopamine fluorescence in the living cell sensitively, and separately from emission of other fluorescent species. When instrumental arrangement and time-resolved spectral analysis can make it possible to solve such problems, direct visualization of the secretion process of individual cells will be achieved by the laser-induced native fluorescence imaging microscopy, without using any additional fluorescent probes. This quantitative imaging technique will provide a useful noninvasive approach for the study of dynamic cellular changes and the understanding of the molecular mechanisms of information transporting processes.

Paper Details

Date Published: 10 May 2001
PDF: 9 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426738
Show Author Affiliations
Michiaki Mabuchi, Kyoto Univ. (Japan)
Junichi Shimada, Yosanoumi Hospital and Kyoto Prefectural Univ. of Medicine (Japan)
Koichi Okamoto, Kyoto Univ. (Japan)
Yoichi Kawakami, Kyoto Univ. (Japan)
Shigeo Fujita, Kyoto Univ. (Japan)
Kazumi Matsushige, Kyoto Univ. (Japan)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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