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Proceedings Paper

Genetically designed biosensing systems for high-throughput screening of pharmaceuticals, clinical diagnostics, and environmental monitoring
Author(s): Brett Romain Wenner; Phillip Douglass; Suresh Shrestha; Bethel V. Sharma; Siyi Lai; Marc J. Madou; Sylvia Daunert
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Paper Abstract

The genetically-modified binding proteins calmodulin, the phosphate binding protein, the sulfate binding protein, and the galactose/glucose binding protein have been successfully employed as biosensing elements for the detection of phenothiazines, phosphate, sulfate, and glucose, respectively. Mutant proteins containing unique cysteine residues were utilized in the site-specific labeling of environment-sensitive fluorescent probes. Changes in the environment of the probes upon ligand-induced conformational changes of the proteins result in changes in fluorescence intensity.

Paper Details

Date Published: 10 May 2001
PDF: 12 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426736
Show Author Affiliations
Brett Romain Wenner, Univ. of Kentucky (United States)
Phillip Douglass, Univ. of Kentucky (United States)
Suresh Shrestha, Univ. of Kentucky (United States)
Bethel V. Sharma, Univ. of Kentucky (United States)
Siyi Lai, The Ohio State Univ. (United States)
Marc J. Madou, The Ohio State Univ. (United States)
Sylvia Daunert, Univ. of Kentucky (United States)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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