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Proceedings Paper

Multispectral excitation of time-resolved fluorescence of biological compounds: variation of fluorescence lifetime with excitation and emission wavelengths
Author(s): Jean-Michel I. Maarek; Sungheon Kim
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Paper Abstract

We investigated the time-resolved fluorescence spectra of tissue structural proteins elastin and collagen I for different excitation wavelengths to assess the usefulness of multispectral excitation for characterization of fluorescent structural proteins in tissue. Laser excitation pulses (4 ns FWHM) at wavelengths between 337 nm and 422 nm were directed to the samples with a multifiber fluorescence probe. The fluorescence emission was measured with a photomultiplier and deconvoluted from the emission pulse with an algorithm based on the Laguerre expansion of kernels technique. A multiexponential approximation of the intrinsic fluorescence decay was computed using a global approach in which the decay constants were held fixed across wavelengths of emission.

Paper Details

Date Published: 10 May 2001
PDF: 8 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426734
Show Author Affiliations
Jean-Michel I. Maarek, Univ. of Southern California (United States)
Sungheon Kim, Univ. of Southern California (United States)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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