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Proceedings Paper

Fluorescence lifetime attachment LIFA
Author(s): Cornelius J. R. van der Oord; Karel W. J. Stoop; Lambertus K. van Geest
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Paper Abstract

We present the Lambert Instruments Fluorescence Lifetime Attachment LIFA. LIFA enables easy to use and affordable microscopy and macroscopic FLIM. The system implements the homodyne detection scheme for measuring the fluorescence lifetime in each pixel of the image. The microscopy system features an ultra bright LED illuminator, the LI-(mu) Cam intensified CCD camera a high frequency signal generator. The illuminator replaces the excitation light source of a standard fluorescence microscopy, while the LI-(mu) CAM intensified CCD camera is attached to the photo-port. Both the illuminator and the intensifier are modulated at a frequency up to 100 MHz at a series of phase differences. The lifetime image is calculated from the series of images on a personal computer.

Paper Details

Date Published: 10 May 2001
PDF: 4 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426731
Show Author Affiliations
Cornelius J. R. van der Oord, Lambert Instruments (Netherlands)
Karel W. J. Stoop, Lambert Instruments (Netherlands)
Lambertus K. van Geest, Lambert Instruments (Netherlands)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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