Share Email Print
cover

Proceedings Paper

New approach to fluorescence lifetime sensing based on molecular distributions
Author(s): Olaf J. Rolinski; David J. S. Birch; Lydia J. McCartney; John C. Pickup
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Fluorescence resonance energy transfer (FRET) from donor to acceptor molecules is one of the most powerful techniques for monitoring structure and dynamics. This is because FRET has a strong spatial dependence with angstroms resolution. This dependence includes the simplest case of a random distribution of acceptors for which an analytical solution exists for the fluorescence impulse response I(t). However, in general the acceptor distribution function p(r) is not random and a unique solution cannot be found for I(t). In many important applications of FRET eg in proteins, the simple random treatment is quite inappropriate and yet the information concerning conformation changes is preserved in p(r). One approach, which as been applied to the problem of determining p(r), is to make some assumptions as to its form eg Gaussian and then try to use this to describe I(t).

Paper Details

Date Published: 10 May 2001
PDF: 11 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426725
Show Author Affiliations
Olaf J. Rolinski, Univ. of Strathclyde (United Kingdom)
David J. S. Birch, Univ. of Strathclyde (United Kingdom)
Lydia J. McCartney, Guys Hospital/Guy's, King's and St. Thomas' School of Medicine (United Kingdom)
John C. Pickup, Guys Hospital/Guy's, King's and St. Thomas School of Medicine (United Kingdom)


Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

© SPIE. Terms of Use
Back to Top