Share Email Print
cover

Proceedings Paper

Morphometric comparison of the acute rabbit and pig corneal response to 1540-nm laser light following ex-vivo exposure
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Single pulse, 1540 nm laser light with a pulse width of 1 microsecond altered the morphologic appearance of explant rabbit and pig corneas following ex vivo exposure. Using digital images of the post-exposure corneas projected onto a measuring grid, we could accurately locate the relative position of the circular laser lesion in the embedded tissue. This allowed us to section through the lesion with micrometer precision and accurately resolve the inside edge, middle and outside edge of the laser lesion. All tissue sections used for morphometric analysis were taken through the middle of the lesion. Several features of the response to laser exposure may reflect species-specific tissue differences. The rabbit corneal epithelium showed a homogeneous coagulative necrosis with a distinct demarcation between necrotic and normal epithelium. The pig epithelium also showed a distinct demarcation between necrotic and normal epithelium, however, there were several remarkable differences in the tissue response between the two species including coagulative necrosis pattern and nuclear morphology. These changes suggested a different and less severe response of the pig epithelium to the laser light when compared to the rabbit epithelium.

Paper Details

Date Published: 17 May 2001
PDF: 5 pages
Proc. SPIE 4246, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment, (17 May 2001); doi: 10.1117/12.426718
Show Author Affiliations
Thomas E. Eurell, Univ. of Illinois/Urbana-Champaign (United States)
William P. Roach, Uniformed Services Univ. of the Health Sciences (United States)
Thomas E. Johnson, Uniformed Services Univ. of the Health Sciences (United States)


Published in SPIE Proceedings Vol. 4246:
Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment
Bruce E. Stuck; Michael Belkin, Editor(s)

© SPIE. Terms of Use
Back to Top