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Proceedings Paper

Morphological slice-based counting of illuminated 3D bodies
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Paper Abstract

This paper presents a simulation toolbox for counting illuminated 3D bodies. The current model is limited to illuminated balls but can be extended to other illuminated bodies. Upon simulation of a set of balls in space, horizontal slices are taken to provide a stack of 2D gray- scale images. Based on these images, a morphological algorithm estimates the number of balls. Construction of the toolbox has been motivated by the need to count spots in FISH images to test for elevated gene copy numbers. The toolbox facilitates analysis of various algorithm parameters based on the distribution of the 3D bodies. These include the number of slices and various settings for the morphological filters composing the algorithm.

Paper Details

Date Published: 8 May 2001
PDF: 7 pages
Proc. SPIE 4304, Nonlinear Image Processing and Pattern Analysis XII, (8 May 2001); doi: 10.1117/12.424988
Show Author Affiliations
Artyom M. Grigoryan, Univ. of Texas at San Antonio (United States)
Edward R. Dougherty, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 4304:
Nonlinear Image Processing and Pattern Analysis XII
Edward R. Dougherty; Jaakko T. Astola, Editor(s)

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