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Proceedings Paper

High-throughput proteomics
Author(s): Scott A. Lesley; Marc Nasoff; Andreas Kreusch; Glen Spraggon
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Paper Abstract

Proteomics has become a major focus as researchers attempt to understand the vast amount of genomic information. Protein complexity makes identifying and understanding gene function inherently difficult. The challenge of studying proteins in a global way is driving the development of new technologies for systematic and comprehensive analysis of protein structure and function. We are addressing this challenge through instrumentation and approaches to rapidly express, purify, crystallize, and mutate large numbers of human gene products. Our approach applies the principles of HTS technologies commonly used in pharmaceutical development. Genes are cloned, expressed, and purified in parallel to achieve a throughput potential of hundreds per day. Our instrumentation allows us to produce tens of milligrams of protein from 96 separate clones simultaneously. Purified protein is used for several applications including a high-throughput crystallographic screening approach for structure determination using automated image analysis. To further understand protein function, we are integrating a mutagenesis and screening approach. By combining these key technologies, we hope to provide a fundamental basis for understanding gene function at the protein level.

Paper Details

Date Published: 16 April 2001
PDF: 10 pages
Proc. SPIE 4264, Genomics and Proteomics Technologies, (16 April 2001); doi: 10.1117/12.424595
Show Author Affiliations
Scott A. Lesley, Novartis Research Foundation (United States)
Marc Nasoff, Novartis Research Foundation (United States)
Andreas Kreusch, Novartis Research Foundation (United States)
Glen Spraggon, Novartis Research Foundation (United States)

Published in SPIE Proceedings Vol. 4264:
Genomics and Proteomics Technologies
Ramesh Raghavachari; Weihong Tan, Editor(s)

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