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Proceedings Paper

Automation of DNA extraction from food and plants using MagneSil paramagnetic particles
Author(s): Rex M. Bitner; Susan C. Koller
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Paper Abstract

MagneSilTM paramagnetic particles allow the flexibility ofautomating the isolation ofDNA from as little as 20mg ofplant material to as much as 500 grains ofvegetable oil for use in testing for DNA sequences from genetically modified organisms (GMO), or plant breeding applications such as random amplification polymorphism detection (RAPD) or polymerase chain reaction (PCR). Given the wide variety of plant materials, foods and highly processed food ingredients that require testing, the purification system must be both scalable and flexible in its ability to purify DNA from such a wide array ofsample types. The procedures used in these purification systems are similar to other methods used for the walkaway automation ofplasmid purification and DNA sequencing reaction cleanup used in genomics applications, as well as DNA purification ofDNA from PCR reactions used for genetic interogations or DNA immobilizations. These purification systems can be used with a variety ofrobotic workstations in 96 well formats.

Paper Details

Date Published: 16 April 2001
PDF: 8 pages
Proc. SPIE 4264, Genomics and Proteomics Technologies, (16 April 2001); doi: 10.1117/12.424594
Show Author Affiliations
Rex M. Bitner, Promega Corp. (United States)
Susan C. Koller, Promega Corp. (United States)


Published in SPIE Proceedings Vol. 4264:
Genomics and Proteomics Technologies
Ramesh Raghavachari; Weihong Tan, Editor(s)

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