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Proceedings Paper

Ultrasensitive DNA and protein analysis using molecular beacon probes
Author(s): Hua Jane Lou; Xiaohong Fang; Lisa Hilliard; Xiaojing Liu; Jianwei Jeffrey Li; Shannon Kelley; Sheldon Schuster; Weihong Tan
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Paper Abstract

Molecular beacons (MBs) are novel nucleic acid hairpin-shaped probes that brightly fluoresce when they are bound to their targets and have a significant advantage over conventional nucleic acid probes. We have developed ultrasensitive MB DNA biosensors, including a fiber optic evanescent wave sensor and a submicrometer optical fiber sensor. The fiber optic sensors are rapid, stable, reproducible, regenerable and have the capability for one base mismatch discrimination. The sensors have been applied for both detection of DNA and specific mRNA sequences. A sensor array has also been demonstrated for multiple target identification. A microwell array methodology has been developed for ultrasmall amount DNA analysis, and can detect 50 DNA molecules in each well. Our study has also extended the applications of MBs from pure nucleic acid hybridization to DNA-protein interactions. These recent advancements of ultrasensitive DNA/protein analysis by molecular beacon probes provide a powerful tool for genomics and proteomics studies, molecular diagnoses of diseases, and new drug development.

Paper Details

Date Published: 16 April 2001
PDF: 7 pages
Proc. SPIE 4264, Genomics and Proteomics Technologies, (16 April 2001); doi: 10.1117/12.424590
Show Author Affiliations
Hua Jane Lou, Univ. of Florida (United States)
Xiaohong Fang, Univ. of Florida (United States)
Lisa Hilliard, Univ. of Florida (United States)
Xiaojing Liu, Univ. of Florida (United States)
Jianwei Jeffrey Li, Univ. of Florida (United States)
Shannon Kelley, Univ. of Florida (United States)
Sheldon Schuster, Univ. of Florida (United States)
Weihong Tan, Univ. of Florida (United States)

Published in SPIE Proceedings Vol. 4264:
Genomics and Proteomics Technologies
Ramesh Raghavachari; Weihong Tan, Editor(s)

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