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Proceedings Paper

Joint optimization of lighting and sensing for the CCD-type DNA chip scanner
Author(s): Jong Dae Kim; Jin-Hee Kim; Jongwon Kim
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Paper Abstract

While the DNA chip is going to be prevalent for the medical diagnostics and drug development, its analysis tool such as a chip scanner need to be optimized. To obtain the guideline for the optimized CCD-based scanner, this paper addresses the minimum requirements for the selected CCD-based detector under the chosen light condition. The scope of our experiments was focused on the scanning performance rather than the design of the chip scanner itself. Through the gray level analysis of the target spot and background, we could check the possibility of the CCD-based detector and the lighting lamp to adopt them as a low price scanner. A quality measure was investigated with two levels of lighting power and the various integration times. For the CCD-based detector and the Xenon lighting system, we evaluated the image performance with the developed quality measure. We define a background and a sample area mask manually to investigate the statistics of the images captured by the scanner. The developed system is composed with a medium price CCD camera and a zenon lamp lighting system. The developed system has reasonable performance for the scanned image. The risk ofphotobleaching was not shown in our experiments.

Paper Details

Date Published: 16 April 2001
PDF: 8 pages
Proc. SPIE 4264, Genomics and Proteomics Technologies, (16 April 2001); doi: 10.1117/12.424588
Show Author Affiliations
Jong Dae Kim, Hallym Univ. (South Korea)
Jin-Hee Kim, BioMedLab Co., Ltd. (South Korea)
Jongwon Kim, BioMedLab Co., Ltd. (South Korea)

Published in SPIE Proceedings Vol. 4264:
Genomics and Proteomics Technologies
Ramesh Raghavachari; Weihong Tan, Editor(s)

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