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Proceedings Paper

Combined confocal and spectroscopic TPE architecture for the identification of single fluorescent molecules
Author(s): Giuseppe Chirico; Fabio Cannone; Francesca Olivini; Sabrina Beretta; Giancarlo Baldini; Alberto Diaspro; Mauro Robello
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Paper Abstract

We have combined a confocal laser scanning head modified for TPE with some spectroscopic modules to study single molecules and molecular aggregates of rhodamine 6G and labeled proteins on glass substrates. The fluorescence intensity of the spots occurs at definite values that are multiples of a reference signal and extrapolate to the background level measured on the images. These properties suggest that these spots arise from single rhodamines. The discrete character of the intensity distribution can therefore be used as a simple, quantitative tool to discriminate between single molecules and molecular aggregates on single snapshots. These studies have been performed by using a combined confocal and spectroscopic architecture realized for two-photon excitation.

Paper Details

Date Published: 24 April 2001
PDF: 7 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424583
Show Author Affiliations
Giuseppe Chirico, INFM and Univ. of Milano Bicocca (Italy)
Fabio Cannone, INFM and Univ. of Milano Bicocca (Italy)
Francesca Olivini, INFM and Univ. of Milano Bicocca (Italy)
Sabrina Beretta, INFM and Univ. of Milano Bicocca (Italy)
Giancarlo Baldini, INFM and Univ. of Milano Bicocca (Italy)
Alberto Diaspro, INFM (Italy)
Mauro Robello, INFM (Italy)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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