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Proceedings Paper

Two-photon confocal microscopy in wound healing
Author(s): Fernando A. Navarro; Peter T. C. So; Antoine Driessen; Nina Kropf; Christine S. Park; Juan C. Huertas; Hoon B. Lee; Dennis P. Orgill
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Paper Abstract

Advances in histopathology and immunohistochemistry have allowed for precise microanatomic detail of tissues. Two Photon Confocal Microscopy (TPCM) is a new technology useful in non-destructive analysis of tissue. Laser light excites the natural florophores, NAD(P)H and NADP+ and the scattering patterns of the emitted light are analyzed to reconstruct microanatomic features. Guinea pig skin was studied using TPCM and skin preparation methods including chemical depilation and tape striping. Results of TPCM were compared with conventional hematoxylin and eosin microscopy. Two-dimensional images were rendered from the three dimensional reconstructions. Images of deeper layers including basal cells and the dermo-epidermal junction improved after removing the stratum corneum with chemical depilation or tape stripping. TCPM allows good resolution of corneocytes, basal cells and collagen fibers and shows promise as a non-destructive method to study wound healing.

Paper Details

Date Published: 24 April 2001
PDF: 14 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424579
Show Author Affiliations
Fernando A. Navarro, Brigham and Women's Hospital (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Antoine Driessen, Brigham and Women's Hospital (United States)
Nina Kropf, Brigham and Women's Hospital (United States)
Christine S. Park, Brigham and Women's Hospital (United States)
Juan C. Huertas, Brigham and Women's Hospital (United States)
Hoon B. Lee, Brigham and Women's Hospital (United States)
Dennis P. Orgill, Brigham and Women's Hospital (United States)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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