Share Email Print
cover

Proceedings Paper

Two-photon investigation of calcium dynamics in dendritic spines during motility
Author(s): Ania Majewska; Ayumu Tashiro; Rafael Yuste
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Dendritic spines receive most excitatory inputs in the CNS and compartmentalize calcium. Spines also undergo rapid morphological changes, although the function of this motility is still unclear. We have investigated the effect of spine movement on spine calcium dynamics with two-photon photobleaching of enhanced Green Fluorescent Protein (EGFP) and calcium imaging of action potential-elicited transients in spines from layer 2/3 pyramidal neurons in mouse visual cortex slices. The elongation or retraction of the spine neck during spine motility alters the diffusional coupling between spine and dendrite and significantly changes calcium decay kinetics in spines. Our results demonstrate that the spine's ability to compartmentalize calcium is constantly changing.

Paper Details

Date Published: 24 April 2001
PDF: 12 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424576
Show Author Affiliations
Ania Majewska, Columbia Univ. (United States)
Ayumu Tashiro, Columbia Univ. (United States)
Rafael Yuste, Columbia Univ. (United States)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top