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Proceedings Paper

New long-wave and highly efficient two-photon fluorophores for multiphoton microscopy
Author(s): Raz Gvishi; Garry Berkovic; Zvi Kotler; P. Krief; J. Y. Becker; Vladimir Khodorkovsky
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Paper Abstract

An important ingredient in improving Multi Photon Laser Scanning Microscopy, MPLSM, is the development of efficient exogenous two-photon fluorescent (TPF) probes. Here we report on a new class of two-photon fluorophores, specifically designed in order to maximize their efficiency in potential MPLSM applications. The fluorophores possess a symmetric Donor-Acceptor-Donor (D-n-A-n-D) structure with varying conjugation length and have strong donors and acceptors. We have studied the two-photon excitation (TPE) properties of these fluorophores and found the following properties: (1) Very large two-photon absorption coefficients (6 > 1000 GM); (2) Peak TP excitation wavelength which are strongly shifted to the red ((lambda) 1 micrometer); (3) Large fluorescence quantum efficiency; (4) Large Stokes shifts of the fluorescence bands. These properties make them particularly suitable for imaging thicker samples, relying on the large improvement in TPE cross-sections and the reduced attenuation at both the excitation and emission wavelengths. We also describe TPE fluorescence anisotropy experiments revealing the tensorial shape of the fluorophores.

Paper Details

Date Published: 24 April 2001
PDF: 7 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424574
Show Author Affiliations
Raz Gvishi, Soreq Nuclear Research Ctr. (Israel)
Garry Berkovic, Soreq Nuclear Research Ctr. (Israel)
Zvi Kotler, Soreq Nuclear Research Ctr. (Israel)
P. Krief, Ben-Gurion Univ. of the Negev (Israel)
J. Y. Becker, Ben-Gurion Univ. of the Negev (Israel)
Vladimir Khodorkovsky, Ben-Gurion Univ. of the Negev (Israel)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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