Share Email Print
cover

Proceedings Paper

Application of two-photon microscopy to elucidate oleic-acid-induced changes in microscale transdermal transport processes
Author(s): Betty Yu; Chen-Yuan Dong; Peter T. C. So; Daniel Blankschtein; Robert Langer
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In a novel application of two-photon scanning fluorescence microscopy (TPM), three-dimensional spatial distributions of the hydrophilic and hydrophobic fluorescent probes, sulforhodamine B (SRB) and rhodamine B hexyl ester (RBHE), in excised full-thickness human cadaver skin were visualized and quantified. These findings utilizing TPM demonstrate that, in addition to providing three-dimensional images that clearly delineate probe distributions in the direction of increasing slun depth, the subsequent quantification of these images provides additional important insight into the mechanistic changes in transdermal transport underlying the visualized changes in probe distributions across the slun.

Paper Details

Date Published: 24 April 2001
PDF: 14 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424557
Show Author Affiliations
Betty Yu, Massachusetts Institute of Technology (United States)
Chen-Yuan Dong, Massachusetts Institute of Technology (Taiwan)
Peter T. C. So, Massachusetts Institute of Technology (United States)
Daniel Blankschtein, Massachusetts Institute of Technology (United States)
Robert Langer, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top