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Proceedings Paper

Observation of real-time interactions of Bcl-2 family members during apoptosis
Author(s): Brian Herman; Victoria Frohlich; Ming Qiu; Akiyuki Takahashi
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Paper Abstract

Apoptosis is a physiological process of cell death resulting from an intricate cascade of sequential protein-protein interactions. Using donor and acceptor mutant GFP fusion constructs, we have monitored the interaction between specific pro- and anti-apoptotic members of the Bcl-2 family with each other as well as proteins located in the outer mitochondrial membrane, as current hypotheses regarding apoptosis suggest that interaction of Bcl-2 family members with each other, or with other mitochondrial membrane proteins, regulates apoptosis. Our data indicate that specific interactions between pro- and anti-apoptotic Bcl-2 family members do occur in situ in the mitochondrial membrane, are altered during apoptosis and regulate cellular sensitivity to apoptosis. These findings are the first to demonstrate real time protein-protein interactions in situ at the level of individual mitochondria.

Paper Details

Date Published: 24 April 2001
PDF: 8 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424549
Show Author Affiliations
Brian Herman, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Victoria Frohlich, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Ming Qiu, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Akiyuki Takahashi, Univ. of Texas Health Science Ctr. at San Antonio (United States)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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