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Proceedings Paper

Multisite two-photon imaging of neurons on multielectrode arrays
Author(s): Steve M. Potter; Natalia Lukina; Kenneth J. Longmuir; Yan Wu
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Paper Abstract

We wish to understand how neural systems store, recall, and process information. We are using cultured networks of cortical neurons grown on microelectrode arrays as a model system for studying the emergent properties of ensembles of living neurons. We have developed a 2-way communication interface between the cultured network and a computer- generated animal, the Neurally Controlled Animat. Neural activity is used to control the behavior of the Animat, and 2- photon time-lapse imaging is carried out in order to observe the morphological changes that might underlie changes in neural processing. The 2-photon microscope is ideal for repeated imaging over hours or days, with submicron resolution and little photodamage. We have designed a computer-controlled microscope stage that allows imaging several locations in sequence, in order to collect more image data. For the latest progress, see:

Paper Details

Date Published: 24 April 2001
PDF: 7 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424542
Show Author Affiliations
Steve M. Potter, California Institute of Technology (United States)
Natalia Lukina, California Institute of Technology (United States)
Kenneth J. Longmuir, Univ. of California/Irvine (United States)
Yan Wu, California Institute of Technology (United States)

Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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