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Proceedings Paper

Nonlinear multimodality spectromicroscopy: multiphoton fluorescence, SHG, and THG of biological specimen
Author(s): Ping Chin Cheng; Chi-Kuang Sun; Bai-Ling Lin; Fu-Jen Kao; Shi-Wei Chu
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Paper Abstract

The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry at high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, but also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG

Paper Details

Date Published: 24 April 2001
PDF: 6 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424541
Show Author Affiliations
Ping Chin Cheng, SUNY/Buffalo (United States)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
Bai-Ling Lin, Institute of Molecular Biology (Taiwan)
Fu-Jen Kao, National Sun Yat-Sen Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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