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Proceedings Paper

Multiexcitation fluorescence and reflectance spectroscopy as a real-time guide to biopsy in vivo
Author(s): Markus G. Mueller; Irene Georgakoudi; Adam Wax; Ramachandra R. Dasari; Christian Jost; Michael B. Wallace; Michael S. Feld
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Paper Abstract

A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeC1 excimer laser, creating laser excitation from 308 nm to 505nm, which is used to excite tissue fluorescence. A xenon-flash lamp is employed to obtain the reflectance spectrum of the corresponding tissue spot. Eleven fluorescence spectra and one white light diffuse reflectance spectrum are collected in vivo in 0.2 seconds and can be potentially analyzed in real time to obtain diagnostic information. The instrument can be used for early cancer detection. The measured fluorescence and reflectance spectra are used to model the intrinsic fluorescence of the tissue, which gives important insight into its biochemical composition (tryptophan, collagen, elastin, NADH, etc.) and metabolic state. Diffuse reflectance provides information about tissue morphology, in terms of the scatterhig and absorption coefficient. By subtracting the diffusely reflected component from the measured reflectance, light scattering spectroscopic (LSS) information due to single backscattering from epithelial cell nuclei can be obtained. LSS provides information about the size distribution of cell nuclei. The parameters give diagnostic information, which can be used to guide the physician in real time while taking biopsies of invisible tissue abnormalities.

Paper Details

Date Published: 16 April 2001
PDF: 8 pages
Proc. SPIE 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, (16 April 2001); doi: 10.1117/12.424525
Show Author Affiliations
Markus G. Mueller, Massachusetts Institute of Technology (United States)
Irene Georgakoudi, Massachusetts Institute of Technology (United States)
Adam Wax, Massachusetts Institute of Technology (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Christian Jost, Medical Univ. of South Carolina (United States)
Michael B. Wallace, Medical Univ. of South Carolina (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)


Published in SPIE Proceedings Vol. 4261:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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