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Proceedings Paper

Light-induced fluorescence for pulpal diagnosis
Author(s): Arata Ebihara D.D.S.; Lih-Huei L. Liaw; Tatiana B. Krasieva; Petra B. B. Wilder-Smith
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Paper Abstract

A direct non-histological means of pulpal diagnosis remains elusive to clinical practice. Clinical vitality testing remains limited to electric, thermal criteria, or laser Doppler flowmetry. The goal of these investigations was to determine the feasibility of using light-induced fluorescence as a non-invasive modality for pulpal evaluation. Such a capability would, for example, permit expanded use of pulpotomy/pulpectomy techniques. Clinically healthy and diseased human extirpated pulpal tissues were used in this study. After excision, they were rapidly frozen and standard cryosections prepared. Measurement of tissue excitation/emission characteristics was performed using spectrographic analysis. A low-light level fluorescence microscopy system was then used to image autofluorescence localization and intensity at optimal excitation/detection parameters. Excitation/detection parameters used in this study included 405/605, 405/635, 405/670, 440/550, and 440/635. Autofluorescence intensities in healthy tissues were significantly stronger than those in diseased tissues at optimal parameters. It is postulated that autofluorescence characteristics are related to pathology- related structural changes in the pulp. This work provides the basis for further investigation into the relation between autofluorescence, histology and clinical symptoms.

Paper Details

Date Published: 27 April 2001
PDF: 6 pages
Proc. SPIE 4249, Lasers in Dentistry VII, (27 April 2001); doi: 10.1117/12.424495
Show Author Affiliations
Arata Ebihara D.D.S., Beckman Laser Institute and Medical Clinic and Tokyo Medical and Dental Univ. (Japan)
Lih-Huei L. Liaw, Beckman Laser Institute and Medical Clinic (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Petra B. B. Wilder-Smith, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 4249:
Lasers in Dentistry VII
Peter Rechmann D.D.S.; Daniel Fried; Thomas Hennig, Editor(s)

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